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Cambrex endothelial basal cell medium-2 (ebm-2)
Endothelial Basal Cell Medium 2 (Ebm 2), supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endothelial+basal+cell+medium-2+(ebm-2)/endothelial+basal+medium/pm40203787-82-0-8
Average 90 stars, based on 1 article reviews
endothelial basal cell medium-2 (ebm-2) - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Molecular Chaperone Receptors
Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were maintained in Endothelial Basal Medium-2 (EBM-2) supplemented with Clonetics TM SingleQuot® (Cambrex/Biowittaker).

Article Title: Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action
Article Snippet: Cells were grown in an 7 endothelial cell basal medium (EBM)-2 with EGM-2 singlequots (Cambrex) at 37◦C in an 8 atmosphere containing 5% CO2.

Article Title: Anti-cancer effect of dung beetle glycosaminoglycans on melanoma
Article Snippet: Briefly, cells were grown in an endothelial cell basal medium (EBM)-2 with EGM-2 singlequots (Cambrex) at 37 °C in an atmosphere containing 5% CO 2 .

Article Title: Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice
Article Snippet: After isolation, total EPC (4 × 10 6 cells, cell density 2 × 10 6 cells/cm 2 ) were cultured on fibronectin-coated (10 μg/mL; Sigma, Deisenhofen, Germany) 24-well plates maintained in 0.5 mL endothelial cell basal medium (EBM-2) supplemented with endothelial growth medium SingleQuots (EGM-2 MV; Clonetics, Cambrex, Walkersville, MD) at 37 °C, 5% CO 2 .

Article Title: RETRACTED: Silence of long non-coding RNA UCA1 inhibits hemangioma cells growth, migration and invasion by up-regulation of miR-200c.
Article Snippet: Silence of long non-coding RNA UCA1 inhibits hemangioma cells growth, migration and invasion by up-regulation of miR-200c

Article Title: PCB153-Induced Overexpression of ID3 Contributes to the Development of Microvascular Lesions
Article Snippet: HUVECs (passages 2–10) were maintained in endothelial cell basal medium-2 (EBM-2) supplemented with EGM2-MV SingleQuots (Cambrex).

Article Title: LncRNA SNHG16 drives proliferation, migration, and invasion of hemangioma endothelial cell through modulation of miR‐520d‐3p/STAT3 axis
Article Snippet: Cells were preserved and maintained in Endothelial Basal Medium‐2 (EBM‐2; Cambrex Bio Science, Walkersville, MD, USA) which containing 10% FBS (Invitrogen, Shanghai, China).

Cell Culture:

Article Title: Molecular Chaperone Receptors
Article Snippet: Human Umbilical Vein Endothelial Cells (HUVEC) were maintained in Endothelial Basal Medium-2 (EBM-2) supplemented with Clonetics TM SingleQuot® (Cambrex/Biowittaker).

Article Title: Glycosaminoglycan Derived from Field Cricket and Its Inhibition Activity of Diabetes Based on Anti-Oxidative Action
Article Snippet: Cells were grown in an 7 endothelial cell basal medium (EBM)-2 with EGM-2 singlequots (Cambrex) at 37◦C in an 8 atmosphere containing 5% CO2.

Article Title: Anti-cancer effect of dung beetle glycosaminoglycans on melanoma
Article Snippet: Briefly, cells were grown in an endothelial cell basal medium (EBM)-2 with EGM-2 singlequots (Cambrex) at 37 °C in an atmosphere containing 5% CO 2 .

Article Title: Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice
Article Snippet: After isolation, total EPC (4 × 10 6 cells, cell density 2 × 10 6 cells/cm 2 ) were cultured on fibronectin-coated (10 μg/mL; Sigma, Deisenhofen, Germany) 24-well plates maintained in 0.5 mL endothelial cell basal medium (EBM-2) supplemented with endothelial growth medium SingleQuots (EGM-2 MV; Clonetics, Cambrex, Walkersville, MD) at 37 °C, 5% CO 2 .

Article Title: RETRACTED: Silence of long non-coding RNA UCA1 inhibits hemangioma cells growth, migration and invasion by up-regulation of miR-200c.
Article Snippet: Silence of long non-coding RNA UCA1 inhibits hemangioma cells growth, migration and invasion by up-regulation of miR-200c

Article Title: PCB153-Induced Overexpression of ID3 Contributes to the Development of Microvascular Lesions
Article Snippet: HUVECs (passages 2–10) were maintained in endothelial cell basal medium-2 (EBM-2) supplemented with EGM2-MV SingleQuots (Cambrex).

Article Title: LncRNA SNHG16 drives proliferation, migration, and invasion of hemangioma endothelial cell through modulation of miR‐520d‐3p/STAT3 axis
Article Snippet: Cells were preserved and maintained in Endothelial Basal Medium‐2 (EBM‐2; Cambrex Bio Science, Walkersville, MD, USA) which containing 10% FBS (Invitrogen, Shanghai, China).



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Workflow of materials and methods. (A) Isolation and bioinformatic analysis of secretome from CDCs. (B) Differentiation and treatment of macrophages with S-CDCs (C) Functional assays with conditioned medium from macrophages: (C1) Interaction with <t>endothelial</t> cells and (C2) interaction with cardiac fibroblasts. CDCs: cardiosphere-derived cells; S-CDCs: secretome released by cardiosphere-derived cells. HUVECs: Human umbilical vein endothelial cells. PCF: porcine cardiac fibroblasts. This figure was created with BioRender.com .
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Cambrex endothelial basal cell medium-2 (ebm-2)
Workflow of materials and methods. (A) Isolation and bioinformatic analysis of secretome from CDCs. (B) Differentiation and treatment of macrophages with S-CDCs (C) Functional assays with conditioned medium from macrophages: (C1) Interaction with <t>endothelial</t> cells and (C2) interaction with cardiac fibroblasts. CDCs: cardiosphere-derived cells; S-CDCs: secretome released by cardiosphere-derived cells. HUVECs: Human umbilical vein endothelial cells. PCF: porcine cardiac fibroblasts. This figure was created with BioRender.com .
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Workflow of materials and methods. (A) Isolation and bioinformatic analysis of secretome from CDCs. (B) Differentiation and treatment of macrophages with S-CDCs (C) Functional assays with conditioned medium from macrophages: (C1) Interaction with endothelial cells and (C2) interaction with cardiac fibroblasts. CDCs: cardiosphere-derived cells; S-CDCs: secretome released by cardiosphere-derived cells. HUVECs: Human umbilical vein endothelial cells. PCF: porcine cardiac fibroblasts. This figure was created with BioRender.com .

Journal: Frontiers in Cell and Developmental Biology

Article Title: Influence of secretome from porcine cardiosphere-derived cells on porcine macrophage polarization and their possible implications for cardiac remodeling post-myocardial infarction in vitro

doi: 10.3389/fcell.2025.1601743

Figure Lengend Snippet: Workflow of materials and methods. (A) Isolation and bioinformatic analysis of secretome from CDCs. (B) Differentiation and treatment of macrophages with S-CDCs (C) Functional assays with conditioned medium from macrophages: (C1) Interaction with endothelial cells and (C2) interaction with cardiac fibroblasts. CDCs: cardiosphere-derived cells; S-CDCs: secretome released by cardiosphere-derived cells. HUVECs: Human umbilical vein endothelial cells. PCF: porcine cardiac fibroblasts. This figure was created with BioRender.com .

Article Snippet: HUVECs (ATCC) were cultured in Endothelial Cell Basal Medium (EBM-2, Lonza) supplemented with growth factors (EGM-2 SingleQuotsTM, Lonza) and used at a maximum passage number of five.

Techniques: Isolation, Functional Assay, Derivative Assay